![]() The hierarchical clustering showed a clear separation of the two groups. (D) Heatmap of DEPs between Hypo and Hypo + Caf groups. These proteins could clearly separate the three groups from each other. ![]() (C) Principal component analysis of 15 samples based on the expression of all the proteins. Caffeine neutralized the decrease in dry and wet weight ratio. The dry and wet weight ratio of lung dramatically declines in Hypo group, suggesting the successful mice model of pulmonary edema model. Hypoxia led to pulmonary congestion and alveolar structural destruction, but caffeine suppressed these damages. (A) Images of hematoxylin and eosin–stained lung tissues. Therefore, caffeine could be a potential treatment for HAPE.Ĭaffeine high altitude pulmonary edema (HAPE) mitochondrial mitochondrial quality control proteomics.Ĭopyright © 2023 Tian, Jia, Yan, Jia, Shi, Cui, Chen, Han, Zhao and He.Ĭaffeine alleviates HAPE by acting on mitochondria. Conclusion: Low-dose of caffeine alleviated HAPE by promoting PINK1/parkin-dependent mitophagy and mitochondrial fission to control the mitochondria quality. Caffeine promoted the PINK1/parkin-dependent mitophagy and enhanced mitochondrial fission to maintain the mitochondria quality control process. We found that caffeine blocked the reduction of ATP production and oxygen consumption rate, decreased ROS accumulation, and stabilized mitochondrial membrane potential to protect AT1 cells from oxidative stress damage under hypoxia. The proteomic results of mice lungs indicated that regulating mitochondria might be the mechanism by which caffeine reduced HAPE. Results: Dry and wet weight ratio and HE staining of pulmonary tissues showed that the HAPE model was constructed successfully, and caffeine relieved pulmonary edema. Methods: We combined a series of biological experiments and label-free quantitative proteomics analysis to detect the effect of caffeine on treating HAPE and explore its mechanism in vivo and in vitro. However, it is unclear whether caffeine plays a role in alleviating HAPE. Caffeine is a small molecule compound with antioxidant biological activity used to treat respiratory distress syndrome. Backgrounds: High-altitude pulmonary edema (HAPE) is a life-threatening disease without effective drugs.
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